This investigation was carried out with the intention of expanding our knowledge of ZnO Pluronic F127 sodium alginate. ZnO Pluronic F127 sodium alginate NPs were subjected to X-ray diffraction analysis, which confirmed that they possess a wurtzite hexagonal structure. ZnO nanoparticles have recognisable peaks in their spectra at 446 cm-1, and their FTIR spectra display the sodium alginate functional group as well as the Pluronic F127 overtone bands. In order to determine the morphologies of ZnO Pluronic F127 sodium alginate, a SEM examination was performed on the substance. Both the hydrodynamic sizes and the DLS spectra of ZnO Pluronic F127 chitosan nanoparticles are 181 nanometers. A blue emission peak can be seen at 478 nm in the PL spectra of ZnO Pluronic F127 sodium alginate NPs. This peak is caused by oxygen vacancies as well as other defects. The well-diffusion method was used to reevaluate the effectiveness of ZnO Pluronic F127 sodium alginate NPs and Amoxicillin against Staphylococcus aureus, Klebsiella pneumoniae, Shigella dysenteriae, Bacillus, and Proteus valgaris. A well diffusion apparatus was utilised in order to assess the degree to which nanoparticles were successful in obstructing the development of Candida albicans. When used to treat a human breast cancer cell line (MDA-MB-237), ZnO Pluronic F127 sodium alginate NPs displayed an anticancer activity that was comparable to that of the