Intranasal photobiomodulation is an innovative technology having numerous applications in medicine. This technique has been identified as having therapeutic applications in treating many symptoms related to central nervous system disorders. It has also been used for treating clinical anxiety and depression in adults. Aim- To observe the effect of intranasal photobiomodulation (IPBM) on anxiety levels of pediatric dental patients. Materials and methodology- Thirty children were selected for the study. They were divided equally into two groups [Group 1: (n=15) Placebo group, Group 2: (n=15) Intranasal PBM group]. The children received dental treatment according to a pre-planned schedule for standardization and received IPBM (Group 1) or placebo (Group 2) according to their allotted treatment group. The baseline, 4 week, 8 week and 3 month readings of Raghavendra, Madhuri, and Sujata Pictorial Scale (RMS-PS) readings, Wright’s modification of Frankl’s behavior rating scale (FBRS), Oxygen saturation and pulse rate were obtained. Results- There was a significant reduction in RMS-PS score, Wright’s modification of FBRS, and pulse rate within group 1 and group 2 from initial assessment to last follow-up period. The overall change in FBRS score within group 2 from initial assessment to last follow-up showed significant improvement. Intergroup comparison showed that difference RMS-PS score between two groups was significant after 4 weeks with group 2 showing significantly lesser score. Conclusion- The present study displays positive results for use of IPBM in reducing anxiety and modulating the behavior of pediatric dental patients. Further long term clinical studies are required to explore the applications of IPBM in detail.