The need for organic recycling is justified in the case of poultry waste because after ensuring hygienization there is a chance of obtaining a compost with substantial fertilizer value. The output of poultry manure has expanded as a result of the poultry industries rapid expansion, and in certain situations, this unplanned discharge of manure to the soil has had unfavorable environmental effects. The experiment was carried out within 60 days in 45*30 *18 (L*W*H) composting bin at laboratory condition. The thermophilic phase was reached within 21 days in all the combination, with a range of 240C to 560C. The temperature in the poultry waste and leaf litter combination decrease to ambient temperature at the end of 45 days towards. The Five different combinations of raw material i.e. leaf litter and poultry litter were used in preparation of compost. Physiochemical properties of compost were determined by the standard procedure. The objective of the study was to examine the impact of poultry compost application on different combination like control, 25%, 50%, and 75% and observation of soil health and nutrient levels in crops. The results showed that compost extract provided more nutrients in compost. The composting rate and different time intervals significantly (p < 0.05) affected the results. The seed germination index (GI), which is a necessary in many national standards, is a frequently used indication of compost maturity. Use of aqueous extracts in seed germination assays was examined for the primary phytotoxicity parameters influencing seed germination in compost. The research described in this article shows the suitability of seeds through germination bioassays and phytotoxicity of poultry composts. It also shows use of poultry litter and leaf litter bio compost as a soil nutrient.