Western transfer and dodecyl sodium sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) are two of the most widely used and beloved techniques in cancer research to identify proteins and glycoproteins. Cellular response to a damaging stressor or to unfavorable environmental factors is frequently linked to the induction of heat shock proteins (Hsps). The primary objectives of this study were to determine whether stress-induced Hsp70 could be used to monitor Cyprinus carpio exposure to different soil pollutants, to determine the specificity of pollutants in the kidney, liver, and brain tissues targeted and in Hsp70 induction, and to determine whether dose-response relationships could be established and whether the stress-response observed was specific. The most researched HSPs are HSP70, which is distinguished from the other HSPs by its high sensitivity, abundance, widespread expression, and association with all subcellular compartments. Due to its numerous roles in the equilibrium of all living things and its quick reaction to any chemical stressor, HSP70 is the focus of this study because it is effective in determining the processes involved in environmental pollution and contamination. According to their molecular weight of 70 kDa, heat shock proteins are categorized as HSP70. These proteins have kept their structural consistency from the simplest to the most complex species. They are a member of the chaperone family, which includes proteins with various structures and similar functions.