This study analyzed main phytochemicals produced in vitro. and antioxidant activity of Broccolisps, antimicrobial activity of soya seed as well as the anti-inflammatory effects of Orange SPS. The primary screening of phytochemical Broccolisps showed the availability of flavonoids, tannins, alkaloids, and phenolic chemicals. Antioxidant capacity of Broccolispsdetermined using DPPH assay and the results showed significant antioxidant activity. The extract of soya seed antimicrobial activity was evaluated against the bacterial strains and the results showed moderate to good antibacterial activity. In our research study, the inflammation-fighting capacity of orange sps. Was evaluated by Protein denaturation and HRBC membrane stability assays, and the results had shown potent anti-inflammatory activity. In this research conclusion, the study provides preliminary proof for the potential use of Broccolisps, soya seed, and Orangesps in the development of novel therapeutic agents with antioxidant, antimicrobial, and anti-inflammatory properties.