Herbs and Plants have been principle form of medicine in India since ancient times. Hundred of medicinal plants have been used to cure diseases. Medicinal plants played a dynamic role in manufacturing human health and improving the quality of human life for thousands of years. Medicinal plants have curative properties due to presence of various complex chemical substance of different composition. Medicinal plants have served humans well as valuable components of medicines, flavorings, beverages, cosmetics and dyes. Ficus religiosa is one of the medically important plants belonging to the family of Moraceae. It has been used broadly in Ayurvedic practitioner in India to treat various disease. The present work is an endeavor to compare the Invitro anticancer and cytotoxicity impact from different extracts of Ficus religiosa.