The present research revealed the invitro antioxidant and cytotoxic properties of ethanolic leaf extract of Muntingia calabura. Natural antioxidants, particularly in fruits and vegetables have gained increasing interest among consumers and the scientific assemblage. since epidermiological studies has pointed that predominant consumption of natural antioxidants is associated with a lower risk of cardiovascular disease and cancer. In the present study, the antioxidant property of ethanolic leaf extract of Muntingia calabura was studied by DPPH Assay and ABTS radical scavenging assays. The cytotoxic activity was assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. The results of antioxidant assay in ethanolic leaf extract of M. calabura reported that DPPH assay depicted the IC50 Value of 82.57 µg/ml followed by ABTS assay showed the IC50 Value of 175.97 µg/ml respectively. The cytotoxic activity of ethanolic leaf extract of M. calabura was carried out against SK-MEL cell line and here the percentage of viability decreased in the increase in the concentration of M. calabura extract and reported the IC50 value of 82.57 µg/ml. The ethanolic leaf extract of M. calabura inhibited the viability of SK-MEL cancer cell lines and these reported the anticancer property of ethanolic leaf extract of M. calabura respectively. From the experimental results it was clear that Muntingia calabura leaf extract was affluent in antioxidant polyphenol and possess evidential antioxidant activity. This work further exploits the possibility of phytomedicine over chemotherapy in disease prevention and control. Thus, the consolidation of edible M. calabura leaves into regular diet could forestall the risk of cardiovascular diseases, ageing, inflammations and cancers due to antioxidant compounds present in the leaf extracts.