Background: Hepatocellular carcinoma (HCC) is the most prevalent and deadly malignant tumor worldwide. Phyllanthus emblica (PE) has a long history of usage in traditional medicine for the treatment of cancer, liver illnesses, hyperglycemia, etc. In this study, we examined the anticancer activity of the bark of Phyllanthus emblica on HCC using the HepG2 cell line. Aim: To analyze the cytotoxic activity of the bark of Phyllanthus emblica on the HepG2 cell line. Methods: The MTT test was used to evaluate the cytotoxic potential of HepG2 cells after they had been subjected to varying doses of the extract. The antioxidant effect was measured using different assays like DPPH, ABTS+ , ferric reducing power, and the phosphomolybdenum assay. The extract was subjected to phytochemical analysis and phytochemical estimation like total phenol and flavonoid. Results: It showed that the ethanolic extract of the bark of Phyllanthus emblica exhibited a potent cytotoxic effect on HepG2 with an IC50 of 12.89 µg/ ml, and the extract shown powerful radical scavenging activity with an IC50 value of 53.52 µg/ml, 41.07 µg/ml, 47.33 µg/ml, and 69.85 µg/ml using DPPH, ABTS+ , ferric reducing power and Phosphomolybdenum assay respectively. The bark extract had total phenolic content of GAE 145.89 µg/ml and flavonoid content of quercetin equivalent 80.57 µg/ml. In vitro study of the PE bark showed positive results against the HepG2 cell line. Conclusion: As a result, the PE bark demonstrated antioxidant and anticancer activity. Hence, it has the potential to deliver promising therapeutic approaches against liver cancer.