Phytochemical research is more focused on the urge for alternative natural phytochemicals that can replace synthetic chemical drugs to minimize the side effects. Controlling the side effects during the treatment is one of the challenges facing medical experts. In the last few decades, medicinal plant research based on traditional, natural, and folk medicines in written form or transferred from ancestors has been of interest to many researchers. It is engaged to explore the most essential basic medicinal properties of medicinal plants or other natural resources, such as antioxidant, anti-inflammatory, analgesic, and immunomodulatory properties. The present investigation aimed to assess the in vitro antiinflammatory properties of the whole ethanolic extract of Spirulina platensis from Lonar Lake in Maharashtra, India. Spirulina was cultured in the laboratory using a modified Zarrouk medium. The maximum growth is achieved in modified Zarrouk’s medium at pH 9.2. The algal ethanolic extract was screened for its anti-inflammation activity by using a protein denaturation inhibition assay and an HRBC membrane stabilization assay. Spirulina whole ethanolic extract proved to have anti-inflammatory potential.