Curcuma amada, a prominent aromatic zingiberaceous plant in the Indian subcontinent, was studied using rhizome explants, which provide an attractive environment for bacterial interaction. This work reveals effective Curcuma amada in vitro propagation as well as the isolation, identification, and analysis of bacteria isolated from Curcuma amada using a 16S rRNA-based molecular approach using samples collected from Curcuma amada. The bacterial strain was isolated and characterised using different biochemical assays, and the molecular technique was used to amplify the 16S rRNA gene using appropriate primers. The bacterial strain was identified by comparing the amplified 16S rRNA gene sequence to the sequence in the NCBI sequence database. 16S rRNA sequencing was used for phylogenetic and molecular evolutionary investigations. When the sequences were submitted to the NCBI gene bank database using BLAST, they revealed 99 - 100% maximum identity and excellent similarity to Burkholderi sps. Burkholderi cepacia strain RRE5, Burkholderi cepacia strain RRE3, Burkholderi cepacia strain CG4, and Burkholderi cepacia T-34 gene. We studied extracts of Curcuma amada rhizomes in this work. Burkholderi cepacia isolate was the most active of the isolates tested.