Amylase is a digestive enzyme that is mostly released by the pancreas and salivary glands and is also present in very minute amounts in other tissues. The primary job of amylases is to hydrolyze the glycosidic linkages in molecules of starch, turning them to simple sugars. One of the most common enzymes utilised in industry is amylase. Due to the wide variety of biochemical processes found in microbes, they are the most favoured source of enzymes. The current study concentrated on isolating amylase producers from soil samples from sugarcane and areca plants. Out of the 10 isolates screened for amylolytic activity using the starch-agar plate method, 2 isolates provided a satisfactory clearance zone surrounding the colonies. Gram staining revealed that isolate 1 was gram negative, rod shaped. While isolate 2 was gram positive, rod shaped. Biochemical methods like indole, nitrate, methyl red and catalase were performed to characterize the isolates. Optimum amylase production was monitored by optimum temperature and optimum pH.