One of the most essential and widely used secondary metabolites produced by bacteria, fungus, and Streptomyces is the antibiotic. The soil is the best place to find microorganisms that produce antibiotics. Soil is a diverse supply of bacteria that produce antibiotics because it is complex, heterogeneous and home to a variety of microorganisms. The majority of antibiotics used today are produced in artificial media, isolated from soil microorganisms and extracted. With the use of an antibiotic produced by a bacterium, some human diseases caused by bacteria, fungus, and protozoa can be healed. The antifungal and antibacterial capabilities of fungi like Penicillium and Cephalosporium make them important sources of secondary metabolites and vital for the synthesis of industrial enzymes. Because they are more affordable and effective at stopping the growth of other organisms, the common soil bacteria known as bacillus species have been proven to be beneficial. The population of microorganisms is dependent on a number of characteristics, including the type of soil, temperature, water-holding capacity, oxygen pressure, pH, carbon supply, salt concentration, organic matter, aeration, moisture content, and others.