In the contemporary medicinal field, various bioactive molecules have been found through experimental research on natural extracts from marine species. Mutualistic symbionts help to improve growth and resist disease or environmental stress. The eleven endophytic fungus isolates found in this study were all found in marine algae samples obtained from the Mandapam rocky shores, Rameswaram, and Ramanathapuram districts of Tamil Nadu. The endophytic fungi were recognized based on the morphology of conidial attachments and the fruit body structure. The evolutionary analysis was conducted in MEGA11. The DNA of fungi was isolated by polymerase chain reaction using a thermal cycler. A Blast search of the sequence showed a maximum similarity of 100% with Curvularia geniculate submitted in the gene bank with the accession code OP321580.