The primary aim of the present study was to isolate and identify a number of sea phytoplanktons for biomass production and determination of carbohydrate content. Sea phytoplankton was cultivated in cultured media “Ares-chat” to multiply seeds and cultivated stocks. In the mass media cultivation, isolation and identification of sea plankton was done based on size and some specific characteristics. The measurement of temperature, salinity and pH was also done in the media. The biomass weight of the density of selected phytoplankton was done by gravimetric method and the carbohydrate content was determined by glucose with Luff School method. The isolation results indicate six different types of sea phytoplanktons: Chlorella sp., Dunaliella sp., Tetraselmis chuii, Chaetoceros calcitrans, Chaetoceros gracilis, and Chaetoceros Isocrysis galbana. The highest biomass content 0.34 gL-1 was on Chlorella sp. and the lowest 0.14 gL-1 was on Isocrysis galbana. The carbohydrate content was also varied, the highest 31.99% on Dunaliella sp. and the lowest 4.70% on Chaetoceros gracilis