The process of isolating an extract from Cassia tora (Linn.) leaves. In this work, an extract was isolated using a Soxhlet apparatus from shade-dried fresh leaves, and a percentage yield (3.25% in ethanol) was achieved. The extract of Cassia tora was then subjected to preliminary phyto-chemical screening, which indicated the occurrence of many subordinate metabolites such as glycosides, alkaloids, saponin, triterpenes, tannin, and flavonoid. Wistar albino rats were used to evaluate the obtained fractions (CT1 to CT5) for wound healing activities; this was followed by column chromatography thin layer of the excerpt, during which the Rf charge was calculated to be 0.91, 0.96, and 1.00%. In order to identify the bioactive molecule in the active fraction of the extract, UV, IR, 13C NMR, 1 H NMR, and Mass spectroscopy were used, with the final product being emodin.