Development of multidrug resistant strains of bacteria and fungi is a major problem faced by the mankind and development of plant anti microbial that show broad spectrum against bacteria and fungi is necessary now a days. In the antimicrobial assay carried for E.coli, P.vulgaris, S.aureus, and fungal strains S.cerevisiae and C.albicans with aqueous extracts of Tamarindus indica, Curcuma longa and Citrus limon (Lemon) by using Streptomycin as reference antibiotic for bacteria and pencillin as reference antibiotic for fungal strains. Citrus limon (Lemon) peel extract showed highest activity against S.cerevisiae and C.albicans but T.indica also showed highest activity against S.cerevisiae but not proven to be more effective for C.albicans, E.coli and P.vulgaris. None of the plant extracts proven to be effective against S.aureus but streptomycin is more effective against S.aureus by 48 hrs of incubation.