Cajanus Cajan (Fabaceae) is a multipurpose plant that is mostly used for human consumption. Various portions from the plants are being utilized as sedatives and are used in the treatment of cough, hepatitis, and diabetes in traditional medicine. In this study the alcoholic extracts of the leaves of Cajanus cajan were subjected to phytochemical analysis. Cetyl palmitate and betulinic acids were separated and characterized from the leaves of Cajanus Cajan. The characterization of phytoconstituents was done by FTIR, mass spectra, 1H and 13C NMR analysis. The cytotoxicity of the extracted bioactive phytoconstituents was determined to be over 500 μg/ml using the MTT assay methodology in HepG2 and Huh7 cell lines. In silico docking and an in vitro investigation of betulinic acid against the target enzyme paraoxonase revealed that it has a higher affinity. As both isolated phytoconstituents having increased secretion for paraoxonase , will be potential candidates in control of atherosclerosis.