Cervical cancer is the fourth most prevalent cancer amongst women worldwide and it is the second most common cancer among women in India. Screening is an important preventive measure against cervical cancer which can help in early detection of pre-/cancer lesions. There is very little data on the knowledge, attitude and practices of females about cervical cancer and its screening in South India. Therefore, this cross-sectional study was conducted among 2400 female students employing stratified random sampling for different educational streams at Vignan University, Guntur in India. Just over 50.54% had heard of cervical cancer and 48.12% had heard about the Pap smear. The mean knowledge score was significantly higher among females aged more than 21 years, those belonging to upper middle socio-economic classes and those pursuing post-graduation (p<0.05). 36.41% of the study subjects had adequate knowledge and 75.87% had favourable attitude towards cervical cancer screening and almost 12.5% had a Pap smear test at least once.