Background: Underreporting of Adverse Drug Reactions (ADRs) is a major obstacle tothe successful implementation ofthe Pharmacovigilance Program of India (PvPI). Methods: A cross-sectional survey was conducted at a tertiary care hospital located in Nellore, Andhra Pradesh, India to assess the knowledge, attitude, and practice (KAP) level toward ADR reporting among healthcare professionals (HCPs).A validated, self-administered questionnaire was used to obtain information on socio-demographics, Pharmacovigilance (PV) exposure, ADR reporting preferences, reasons for underreporting, and KAP towards ADR reporting. Aunivariate combined with multivariate logistic regression analysis was used to assess factors associated with KAP toward ADR reporting. Results: Among 396 HCPs, the majority of them showed a positive attitude towards the ADR reporting (254; 64.14%), but very few are having good knowledge (102; 25.76%) and rational practices (97; 24.49%). Pharmacists and academicians were positively associated with good knowledge (AOR 8.56; 95%CI 4.23-18.62), positive attitude (OR 2.84; 95%CI 1.12-12.35), and rational practice (AOR4.13 95%CI 1.94-10.32) towards ADR reporting compared with the doctor. Whereas, nurses (AOR 0.19; 95%CI 0.12-0.74), lab technicians (AOR 0.11; 95%CI 0.01-0.74), and interns (AOR 0.38; 95%CI 0.12-0.93) were negatively associated with the practice of ADR reporting compared with doctors. Advanced age, healthcare experience, PV training, and work in the PV were shown a significant association with KAP towards ADR reporting. Conclusion: Although the majority of HCPs expressed a positive attitude towards ADR reporting, there was a gap in the adequacy of knowledge and practices toward ADR reporting. Nurses and practicing interns have shown a very low KAP toward ADR reporting. The study recommends that PV training programs targeting individual professional needs, and barriers to underreportingcan improve the reporting of ADRs among HCPs