Background: The consumption of Smokeless Tobacco (SLT) among people in India region leads to avoidable oral cancer cases and premature deaths every year. Objectives: To assess the knowledge, attitude, practice and barriers of smokeless tobacco use and cessation during the COVID-19 pandemic in Manipur, India. Materials And Method: The study enrolled a cohort of smokeless tobacco users aged 15-65 years whose data on SLT use and cessation habits were recorded using a self-administered, pre-tested and pre-validated questionnaire containing 28 questions divided into four sections. Questionnaires with ≤1 incomplete responses were excluded from the study. Data was found to be parametric by applying the Shapiro–Wilk test, and further analysis included the independent sample’s t-test and multivariate linear regression using IBM SPSS for Windows, v. 24.0.