Background: The goal Knowledge and awareness about the hazards of radiation is an important subject not only for those who are occupationally situated in radiation zones but also for other healthcare professions as well as those visiting healthcare and diagnostic services. The main objective of the study was to assess the Knowledge of radiation safety among non-radiology healthcare professionals in tertiary hospital. Methodology: A total of 196 participants responded to self-administered questioner. The study revealed that there was poor knowledge about radiation hazard and safety among the healthcare professionals. Very few healthcare workers correctly answered the radiation protection for patients. Result: The mean and standard deviation for assessing the knowledge of radiation hazards among the patients was 5.719±2.271. Only 7.65 % received formal training 7.14 % were educated by means of tutorial, 13.77 % learned from different sources and 21.93% participants had no training and learned from other source. 51.53 % could correctly identify the radiation symbols. 87.75 %of wants to be trained in radiation hazards and safety.