In this paper, the impact of various leadership styles on the organizational culture in higher education institutes of District Mohali, Punjab, has been evaluated. The paper also suggests suitable measures to improve organizational culture in higher education institutions in District Mohali. The relationship between the dimensions of leadership styles (Participative, Laissez Faire, Autocratic and Situational) and Organizational Culture (Autocratic, Bureaucratic, Technocratic and Entrepreneurial) in relation to Higher Educational Institutions in Mohali has been presented. The link between the scales of transformational and transactional leadership style and organizational culture has been demonstrated by this study. Educational institutions should concentrate on utilizing the transformational leadership style to increase employee work satisfaction.