Millionsofpeopleworldwidediefromcancereveryyear.Researchsuggeststhatcancerstemcellsareresponsible for recurrence and resistance. Drug resistance in cancer stem cells is associated withnumerouspathways.OneofthoseisHedgehog(Hh)pathway.Giventhis,forty-fivealreadymarketedanti-cancermoleculeswerechosen and screened for inhibition of the Hh pathway using Hedgehog protein (PDB ID: 4C4M) andSmoothened protein (PDB ID: 4JKV). Preliminary molecular docking analysis was performed to studydrug-receptor interactions using AutoDockVina. Upon conducting the docking analysis, it was observed that the formation of hydrogen bonds was primarily influenced by the presence of hetero atoms such as oxygen and nitrogen in diverse concentrations. Moreover, the benzene ring displayed advantageous pi-pi stacking interactions with several amino acids, resulting in an overall improvement of the docking score.