At present Lixisenatide is the drug been used to treat type 2 diabetes mellitus. Therapeutically is been considered to be best for treatment of diabetes mellitus than other GLP1 receptor antagonists Objectives- To find out gross anatomical malformations due to teratogenic effects of antidiabetic drug Lixisenatide on developing chick. Methods- It was an Interventional study conducted at Department of Anatomy, Santosh Medical College, Ghaziabad. It is confirmed from literature survey/ pilot study that the expected mean ± SD of parameter of control and experimental groups are 27± 5.32 with 32±5.30, with the help of software G* Power analysis for α 5% and Power (1-β) 95.36 %. The effects size is 1.961 and sample size for each group is 28. So we have taken the total sample size 280. Results- Gross anatomical malformation was seen after administration of lixisenatide as compared to control group. The most common anatomical malformation was growth retardation followed by limbs deformity and twisted neck and it was statistically significant (p<0.05) significant decrease in the weights of treated chick embryo compared to the control groups (p<0.05) Conclusion- The data generated in this project shows teratogenic effects like anatomical malformations and histopathological changes due to effects of high dose of Lixisenatide on developing chick embryo in liver organ and these teratogenic effects may be extrapolated to human being.