This study aims to measure changes in oxygen consumption in relation to the amount of natural feed requirements based on artificial intelligence, analyze the relationship of oxygen consumption with the use of natural feeds monitored by artificial intelligence and calculate the appropriate natural feed density for Brachionus plicatilis in meeting daily natural feed requirements in rearing. milkfish larvae. This study used a completely randomized design (RBD) with three treatments and three replications. The treatments tried were Brachionus plicatilis densities of 25 ind/mL, 50 ind/mL, and 75 ind/mL. The number of hatching milkfish larvae used was 2880 larvae with an average body length of 0.376 mm ± 0.02 and reared for seven days. Oxygen consumption parameters were measured using the respirometer bottle method. The results showed that oxygen consumption tended to increase up to a density of 75 ind/mL and decrease again at a density of 50 ind/mL. The best density of Brachionus plicatilis in the study was 50 ind/mL. However, specifically the second to fifth days a density of 25 ind/mL is sufficient and the sixth and seventh days are given at a density of 50 ind/mL