Tomato, an important vegetable crop is being prone to many viral disease, among which Groundnut bud necrosis virus (GBNV) causes huge yield loss. The study conducted in 2021 across diverse tomato fields in Coimbatore District, Tamil Nadu, investigated the prevalence and symptomatology of GBNV. Disease occurrence varied from 9.67% to 30.33% across different villages. Controlled experiments were conducted using infected tomato leaves for controlled sap inoculation in a glasshouse, providing insights into GBNV's distribution and symptoms. Field observations unveiled a symptom progression caused by GBNV, starting from chlorotic ring spots on leaves and developing into necrotic ring spots. Young shoots exhibited necrosis and bud withering, stems and petioles displayed necrotic streaks, and fruits showed chlorotic rings. Early infections resulted in plant wilting and stunted growth, highlighting the virus's impact. A cowpea assay demonstrated GBNV's efficient transmission (100%) and induced chlorotic and necrotic spots on leaves within 4 and 8 days post-inoculation, respectively. These findings aligned with prior research, confirming the virus's virulence and systemic spread. Pathogenicity was successfully established in tomato plants, meeting Koch's postulates. Inoculated plants exhibited symptoms akin to natural infections, including necrotic rings on leaves and stem necrotic streaks. Symptom progression over time and systemic effects further supported GBNV's virulence.