Background: According to recent research, the paracrine substances produced by mesenchymal stem cells (MSCs) appear to be the primary element responsible for the therapeutic action reported in these cells. MSC-CM, also known as the secretome of MSCs, is a rich source of paracrine factors and is being extensively studied for a variety of regenerative therapies, including wound healing and metabolic disorders. Aim of the work: The current review provides the evaluation of Mesenchymal stem cells conditioned media outlining its clinical applications as well as its mechanism of action. Conclusion: In summary, researchers have frequently emphasized the significance of Mesenchymal stem cells conditioned media administration as a new line for treating various diseases.