Mesenchymal stem cells (MSCs) is a form of regenerative medicine that utilizes the body's natural healing mechanism to treat various conditions. MSCs are used to renew and repair diseased or damaged tissue mostly it is used in the treatment of various orthopaedic, cardiovascular, neuromuscular and autoimmune conditions. Stem cells are present in all of us, acting like a repair system for the body. However, the number of stem cells decreases by increasing age due to this the required amount of stem cells are not delivered to the injured area. The main goal of stem cells is to amplify the natural healing process and repair system of the patient's body. In order to develop an effective automatic device that can extract MSCs from bone marrow blood concentration (thick blood) using automatic microcentrifugation and density gradient separator to isolate MSCs from other cell types. This device can isolate MSCs from other cell types with high purity and efficiency. However, this device reduces the risk of contamination of stem cells from bacteria and viruses as compare with manual extraction methods. This automatic device significantly improved the yield and purity of MSCs, and reduced the time and human efforts required in the extraction process.