Celastruspaniculatus(CP), a traditional herb belongs to the family of Celastraceae known for its memory and cognitive enhancing properties. The CP seeds were the extensively studied material, but its metabolomics was still unknown particularly using combined extraction method. Hence the aim of this study is to determine the bioactive compounds of CP seeds using various solvents and by combined extraction method. CP seeds were procured from Kanyakumari, Tamilnadu and subjected to extract preparation with different solvents. After the initial phytochemical analysis, total and combined ethanolic extract of CP were chosen for further study. Gas chromatography-mass spectrometry (GC-MS) and Thin-layer chromatography (TLC) outcomes were analyzed to draw an inference based on variation in its metabolomics. A better understanding of its effectiveness against various medicinal conditions required further research.