An efficient, Sensitive and rapid high-Performance liquid Chromatographic Method with UV detection has been developed for the quantitation of antibacterial medications i.e. ornidazole from human plasma. The chromatographic separation was carried out on RP C18(Waters) column (250×4.6 mm, 10mcm) using isocratic mobile phase, Ammonium Acetate (pH 4(pH 4.0,20 mM): acetonitrile (70:30 % v/v) pumped at a flow rate of 1ml/min. The analyte ornidazole and internal standard tinidazole were monitored at 318 nm. The method is specific and linear over the range of 0.025- 3.00 µg/ml with intraday precision of 1.9169% CV and interday precision of 1.1093% CV. Ornidazole was found stable in plasma during sample processing (autosampler) and 30 days of storage in a freezer. This validated method can be applied for bioavailability/ bioequivalence studies.