An accurate, precise, specific, reliable, and simple reverse phase high performance liquid chromatography method was developed and it validated for simultaneous estimation of there (atenolol, metoprolol, propranolol) with hydrochlorothiazide in pharmaceutical dosage form. The formic acid (0.1%) in water and acetonitrile was used as mobile phase with flow rate of 1 mL min-1. The detection carried out on colum Agilent HPLC 1200 series bridge C18 column (Xbridge C18, size: 250 mm x 4.60 mm, particle size 5 μm) at 228 nm was used. The method was found effective in the concentration range of 10 - 90%. This was applied for quantitative determination of atenolol, metoprolol, propranolol with hydrochlorothiazide in dosage form successfully. The retention times of hydrochlorothiazide, propranolol, metoprolol, and atenolol, were found to be 1.368, 4.123, 6.477, and 7.230 min, respectively. The present RP-HPLC method was validated and was used for the analysis of hydrochlorothiazide, metoprolol, atenolol, and propranolol in combined tablet dosage form (pharmaceutical formulations).