Micro and Nano fabricated cantilever sensor devices have attracted much interest in recent years because of their fast and reliable detection with a high surface-to-volume ratio. Here a novel biosensor was developed based on MEMS technology to detect dengue virus (DENV) in early stages, using cantilever deflection due to antigen (Ag) and antibody (Ab) reaction. To develop the proposed biosensor, a thin layer of prime dengue antibodies (IgM) coated on the active surface of the cantilever after functionalization. After that, exposed to the antigen (DENV1- 4), as it contains a significantly high sensitive biomarker, Non-Structural-1 (NS1) is available at detectable concentrations in inpatient blood during 2-9 days. This research paper uses a novel technique called the “catalytic approach” to enhance biosensor performance. The experimental results proved that cantilever response is good enough to detect NS1 with minimum interference for four serotypes of dengue virus (DENV-1 to DENV-4).