Escherichia coli is well known to be a universal commensal flora in humans as well as in several animal species. Antimicrobial resistance (AMR) has emerged as one of the principal public health problems of the 21st century. This study was undertaken for detection of the antibiotic resistance and Amp C β- lactamase genes for E.coli isolates. Methods: Different clinical samples (urine, diabetic food ulcer, vaginal discharge, burn exudate, stool, sputum, blood, ear swab and CSF) were collected from patients from February to June 2022. Out of 1874 clinical samples, 231 were belonged to E. coli isolates,112 (48.5%) were produce β-lactamase, E.coli were examined for list of antibiotics. Results: All isolates resistance to ampicillin, amoxicillin and amoxicillin/clavulonate, resistance to piperacillin (99.1%), E.coli sensitive for meropenem (100%), etrapenem (96.5%), impenem (95.5%), amikacin (92%) and isepamicin (98.2), According to pAmp C genes, show high prevalence BlCMY (88.5%), BlCIT (57.7%) and BlDHA(42.3%), BlFOX, BlMOX, BlEBC, BlACC were not detected in present study, From 26 pAmp C producing isolates, 1(3.85%) isolates carry 3 types of pAmp C genes, 19(73%) isolates carry 2 types of pAmp C, 5(19.3%) isolates carry 1 isolates and 1 (3.85%) isolate do not carry any of the studied genes. Conclusions: E. coli isolated from various clinical specimens showed differences in antibiotic sensitivity patterns, with high resistance to commonly used antibiotics, high prevalence of BlCMY, BlCIT and BlDHA genes in E.coli isolates and class 1 integron leading cause for E.coli resistance.