The study aimed to synthesize novel compounds that could serve as potent inhibitors of Pseudomonas aeruginosa and Staphylococcus aureus, two of the most common bacteria causing infections in humans. Specifically, the study focused on the synthesis of 3-(3- hydroxypropyl)-7-(4-nitrophenyl)[1,3] thiazolo [4,5-d] pyrimidin-2(3H)-one and its molecular docking studies with the target enzymes of the two bacteria. The results of the study suggest that the synthesized compound could effectively inhibit the growth of P. aeruginosa and S. aureus, making it a promising candidate for further development as a potential antibacterial agent.