Mastishk vriddhi caused death in humans from ancient times to be also known as Glioblastoma multiforme. It is grade IV astrocytoma and appears as star-shaped glial cells in the frontal and temporal lobes of the brain. Temazolamide with radiation therapy to treat Glioblastoma currently but still not succeed completely and exhort threat as drug resistance.DYRK1A and IDH target the cell cycle and manage mitochondrial dysfunction to control oncoprotein 2-hydroxy glutarate—this study structure-based drug design method to screen natural compounds from the Himalayan region.