Nitrogen-fixing endophytic bacteria have been regarded as a potential substitute for chemical nitrogen fertilizers to enhance plant growth and yield. The objective of this study was to isolate and identify endophytic bacteria with diazotrophic properties and abiotic stress tolerance ability from root and stem of kaladana rice grown in Jharkhand. To achieve this goal, endophytic bacteria were extracted from the roots, stems of the plant tissues, and strains that promote plant growth were specifically identified. A total of 11 endophytic bacteria were isolated from the stems and 10 endophytes as diazotrophic nitrogen-fixing bacteria grown in JNFb- media. Further, 4 Out of the isolated bacteria were identified as diazotrophic bacteria using a specific primer set targeting the nifH gene. By performing 16S rDNA sequence analysis, the presence of nifH genes confirmed the existence of four distinct species, namely Bacillus aryabhatti (accession no. OQ426536). Priestia aryabhatti (accession no. OQ422565). Bacillus magaterium (accession no. OQ422490). Staphycoccus hominis (accession no. OQ422489). In addition, the study also investigated the phosphate solubilizing and abiotic stress tolerance activity of the isolated bacteria to explore potential mechanisms for plant growth promotion. Out of the four isolates tested, all four strains exhibited significant levels of phosphate-solubilizing and abiotic tolerance activity. The findings of this study strongly indicate that the identified endophytic diazotrophic bacteria have great potential for effectively enhancing plant growth in sustainable agriculture.