Chronic skin conditions may cause systemic infections. Infectious skin agents may spread via mucosal membranes or skin abrasions. Regional variances in bacteria, antibiotic resistance, and human immune responses make treating critical illnesses challenging. Chronic skin conditions may enhance microbe sensitivity. The aim of the research is to assess the Antimicrobial effect of gel formulations prepared from ethanolic and aqueous extract of Azadirachta indica Linn., commonly known as Neem, extract was tested against Candida albicans, E.coli, B.subtilis, and S.aureus which are the common bacteria causing skin diseases by agar well diffusion method. The formulation numbering F3E, were found most effective among the twelve formulations prepared by using 4% concentration of ethanolic extracts of the plants and formulations numbering F3A, were also found most effective among twelve formulations prepared by using the 4% concentration of aqueous extract of the plants. Azadirachta indica Linn contain phytochemicals, which have therapeutic potential. Ethno-medical usage has shown that plants may heal several skin diseases. The use of plant species Azadirachta indica Linn and their phytoconstituents to treat various skin illnesses is growing.