The present investigation was carried out during 2021-23 in the Department of Fruit Science, Horticultural College and Research Institute, Periyakulam. Sixty mango genotypes were taken for the experiment with the objective to study the morphological and Biochemical behavior of mango genotypes. Wide variability was recorded for different phenological traits. Maximum leaf area, hermaphrodite flower per cent, TSS was recorded by Sindhu (272.95 cm2, 37.11%, 22.700Brix). Maximum male flower per cent was recorded by Pedharasam (79.60%). The Highest acidity and ascorbic acid content was observed by Sothuparai local (1.20%), Mallika(52.08mg100 g-1).Hence,it could be concluded that the cultivar Sindhu, Pedharasam ,Sothuparai local, Mallika can be used in breeding programme to getting maximum leaf area, hermaphrodite flower per cent, TSS, male flower per cent, acidity and ascorbic acid content.