Mycotoxins are natural toxins mainly produced by pathogenic fungi and these are found in plants and consumed crops such as wheat, rice & maize etc and their products. Mycotoxins causes various serious health risk to humans; they can enter human body by contaminated food products. In the present study we analysed using in silico studies the harmful effect of mycotoxins of wheat (Triticum aestivum) on humans utilizing computational tool ProTox-II. In silico toxicity and other toxicity parameters of mycotoxins were analysed using this tool. International authorities EU & USFDA set permissible range of mycotoxins in food based on LD50 values, authorities have categorized mycotoxins into five classes (1-5). Class 1 is fatal and class 5 is categorized harmful for human consumptions. Results obtained were compared to permissible LD50 value, all the predicted mycotoxins of wheat exceeded from permissible range. Aflatoxin B1, Deoxynivalenol (DON) and HT-2 were predicted in class 1 which is fatal to humans. Other mycotoxins, Ophiobolin, Aflatoxin G1 & M1 were found under class 3, and these are also very harmful to humans. Almost all the toxin are neurotoxic and carcinogens. This study clearly states that some mycotoxins are very harmful and can cause severe damage to liver and other organs of humans and they are carcinogen and immunotoxic also. Therefore, a safe approach should be developed to mitigate the contamination of Mycotoxins by inhibiting the infection of plant pathogenic fungi using biological methods.