Curcuma longa L. belonging to family Zingiberaceae is a valuable medicinal plant containing pharmaceutically important curcuminoids (Bisdemethoxy curcumin, Demethoxy curcumin and Curcumin). Plant tissue culture is a leading technique utilized for secondary metabolite production. Nanoparticles find their applications as elicitors for enhancing the production of secondary metabolites from in vitro plant cell organ cultures. This study was conducted to investigate the effect of ZnO, CuO, and Ag nanoparticles on in vitro grown plants of C. longa. The effect of ZnO (30, 60, 120mg/L) CuO (1, 2 and 5mg/L) and Ag (0.5 and 3mg/L) was examined on growth and curcuminoid production. The in vitro shoot cultures were evaluated for biomass increase and secondary metabolite production by augmenting nanoparticles for a period of 40 days. ZnO 60mg/L with MS basal media gave best response for biomass and curcuminoid production after 30 days of inoculation. There was an increase of 10 fold curcuminoid production on 60mg/L ZnO containing media in comparison to control cultures. Ag nanoparticles proved to be better than CuO nanoparticles for growth and curcuminoid production.