Background:Thenexus among urine protein and serum nitric oxide, insulin resistance has beenevaluated in type 2 diabetics by earlier workers. However, we wanted to elicit the value of nitric oxide as a solitary, reliable marker of oxidative stress mediated endothelial dysfunction in nephropathy. Methods:Thestudy subjects (n=150 total; both genders) were those who had visited the clinics of a tertiary health care set up in South India.The groups were segregated: fifty patients with normoalbuminuria (<30 mg/g creatinine), and an equal number of patients each with microalbuminuria (30–299 mg/g creatinine), macroalbuminuria (≥300 mg/g creatinine). Fifty healthy age, gender matched subjects constituted the control group.The duration of T2DM in the experimental group was greater than five years. Results:In comparison to the control group, the magnitude of glycated haemoglobin (HbA1c), insulin resistance and nitric oxide (p<0.05) were statistically significant in the study groups, as a function of albuminuria. Conclusion: It was concluded based onthe correlation analysis of Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), glycated hemoglobin (HbA1c), fasting plasma glucose (FPG) and Postprandial blood glucose (PPBS) with nephropathy markers, namely urinary albumin creatinine ratio (UACR) and eGFR that nitric oxide could serve as a solitary, reliable and objective marker of endothelial dysfunction in oxidative stress related nephropathy. Since insulin resistance and serum nitric oxide are found to be closely related to diabetic nephropathy, they could serve as reliable reference indices for evaluating diabetic nephropathy.