Biodegradation is a promising method for producing hydrogen, a clean and renewable energy source, from organic waste materials. This process involves the use of microorganisms, such as bacteria, to break down organic matter into simpler compounds and produce hydrogen as a biological by-product. The biodegradation of waste materials, such as agricultural residues, food waste, and wastewater, not only produces hydrogen but also provides an eco-friendly solution for waste management. Several factors affect the efficiency of hydrogen production through biodegradation, including the type and concentration of organic matter, the type of micronutrients, pH, and the type and inoculum size of microorganisms used. Therefore, optimization of these factors is crucial for maximizing hydrogen production. In this project, novel strains Bacillus paramycoides and Cereibacter azotoformans were explored for dark and photo fermentation processes to produce biological hydrogen. Various operating parameters were experimented with, including the concentration of glucose and xylose, types of metal ions, and bacterial inoculum sizes for hydrogen generation. The study shows that these operating conditions have a significant effect on the biological hydrogen yield from the fermentation process. In a summary, biodegradation-based hydrogen production offers a promising avenue for producing clean and renewable energy while simultaneously addressing the pressing issue of waste management.