Background: One of the top 10 worldwide public health hazards to humanity, according to WHO, is AMR. The main forces behind the spread of bacteria that are resistant to antibiotics are misuse and overuse of antibiotics. The goal of the current study was to create a successful antimicrobial stewardship programme for tertiary care hospitals establishing incidence of MDR,XDR and PDR. Materials and Methods: This retrospective study was conducted from 1st January 2021 to 31st October 2022. Laboratory data were collected from the medical record section. Results: Of the total clinical sample received 7633; pathogenic bacteria were isolated from 2422 [32%] clinical samples of which 1778 [23%] were gram-negative bacteria. The most prevalent microorganism was Escherichia coli 768 [43%], followed by Klebsiella pneumoniae 474 [27%], Pseudomonas aeruginosa 238 [13%], and Acinetobacter spp. 91[5%], Citrobacter spp. 91[5%], Enterobacter spp.74 [4%], Proteus spp. 28 [2%]. 84 [5%] strains were Carbapenemase producers. 705 [40%] strains were ESBL producers predominantly from UTI and sepsis infections caused by E. coli and Klebsiella pneumoniae. Conclusion: E. coli followed by Klebsiella pneumoniae were the most prevalent reported microorganisms. The rapid rise in MDR cases is regarding and accentuates the essentials for sustained surveillance, accurate diagnostic microbiological laboratory capacity, earlier diagnostic tests, and robust infection prevention and control.