The bimetallic nanoparticles of FeO-Ag were prepared by co-precipitationtechnique.The synthesized bimetallic nanoparticles were characterized by FTIR, UV–Vis Spectrophotometry,Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectrometry (EDAX), antimicrobial activities and Bio film formation. The average diameter was found using DLS characterization. From EDAX studies the percentage of the present elements and morphology of the NP was found using SEM studies. In both anti bacterial and anti fungal study the higher concentration gives good potential activity then the other concentrations. These techniquesconfirmed the formation of the bimetallic nanocomposite and showed that the sizedistribution of the synthesized bimetallic nanoparticles