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ISSN 2063-5346
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OPTIMISATION OF THE EXTRACTION PROCESS AND PARTIAL CHARACTERIZATION OF Β-GLUCAN FROM THE MARINE YEAST DEBARYOMYCES HANSENII.

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G. Vidya Sagar Reddy, Ch. Vijaya
» doi: 10.53555/ecb/2022.11.12.209

Abstract

The cell walls of fungi are made with structural polysaccharides as well as glycoproteins. They are the main source of various glucans. Debaryomyces hansenii yeast cells (15% W/V) was used as the source of β- glucan extraction. 1M NaOH (sodium Hydroxide) / 1M HCl , 1 M NaOH / 1M CH3COOH , 1 M NaOH / 1M H3PO4 combinations were used for optimization of the extraction procedure. Maximum yield of β-glucan from D. hansenii was obtained with a combination of 1M NaoH / 1M H3PO4 extraction method. FT-IR analysis of the extracted β-glucan identified the signature peeks of bonds (1,3) and (1,6)-β-D-glucan linkages.

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