Morphine is one of the most potent opioid analgesics, the World Health Organization (WHO) advises using it but long-term use can result in addiction and physical & psychological dependence. A high performance liquid chromatography (HPLC) method has been developed and validated as per International Conference on Harmonization ICH Q2 (R1) guidelines to determine morphine in distinct seized narcotic evidences. Very few studies are there on seized drug samples, mostly are on biological and pharmaceutical samples because of the complex nature of seized drug samples but its analysis is important to fight against drug trafficking and drug abuse. In this HPLC method, C18 column and photo diode array (PDA) detector at λ = 280 nm is used. Good level of stability and reproducibility is observed in the results with the particular composition of mobile phase used here. Linearity is reported in the concentration range of 0.5–15 μg/mL, and a remarkable correlation coefficient (R2 = 0.9961) is determined which has not been reported earlier. The proposed method does not involve the use of expensive chemicals and shows better acquisition rate. Moreover, simple mobile phase preparation makes the entire procedure incredibly time-efficient making it the method of choice for routine morphine analysis.