Aims: Bacteriophages are a promising alternative to antibiotics in poultry. As more phages are required for phage therapy, ,there is a need for optimization of phage production conditions to produce more Salmonella enterica specific phages for poultry applications. Methodology: An S.enterica SAL-PG bacteriophage isolated in our laboratory was used for optimization studies. The production process was carried out by conventional methods to identify the factors, and the Thaguchi process was used for the optimization of conditions for increased bacteriophage production. With four factors each at three levels L9, experimental runs were carried out