Endophytic fungi are a group of microorganisms that reside within the internal tissues of living plants, exhibiting a communalistic relationship with their host without causing any apparent harm to the latter. The dynamic nature of plant ecosystems is attributed to the modification of species' structure and composition in response to various circumstances across different plant tissues. Certain endophytic fungi have exhibited the potential to serve as a prolific reservoir of innovative bioactive compounds that hold promise for diverse fields such as pharmaceuticals, agriculture, and industrial applications. This research aimed to improve the metabolites generated by endophytic fungus by isolating and growing them under optimal physical and chemical conditions. The different sources of substrates like carbon, nitrogen, inorganic nitrogen, concentration of sodium chloride, pH and temperature levels were provided for the optimum growth condition of Curvularia geniculate to produce secondary metabolites at high yield.