Most of these investigations are performed to determine the toxicity of substances to humans, animals, or the environment; to learn more about the mechanism of hazardous chemicals; or to provide better methods for detecting certain chemically caused effects. In light of OECD recommendations, the current study set out to assess the acute and 28-day repeated oral toxicity of a polyherbal formulation. Animals in an acute oral toxicity study were given a dose of 2000 mg/kg of the herbal mixture and then monitored every 30 minutes for the next 1, 2, and 4 hours. Four groups of six rats each were used to conduct a repeated dose, 28-day oral toxicity investigation. The animals in Group 1 were used as a comparison. Animals in Group II were given a low dose of the herbal mixture (50 mg/kg), whereas those in Group III were given a moderate dose (100 mg/kg), and those in Group IV were given a high dose (200 mg/kg) (orally, once daily, for 28 days).