Bone in the daily activities come in to contact with different type of loads and sometimes become a reason of fracture or failure when it exceeds the value of failure limit. Among these load types, the stresses generated due to shear loading are typically minor in contrast to tensile and compressive stresses, but they can become significant in bones with long lengths. Therefore, it becomes necessary to define the failure criteria during shear so that a necessary measure can be taken to avoid the failure or fracture of a bone. In the current study, two type of specimens with orientations Longitudinal and Transverse to the axis of femur animal bone has been considered to measure the shear properties of a cortical bone using Iosipescu test FEM model. The shear modulus of longitudinal specimen found to be more in comparison to transverse orientated specimen. Also, it has been found that when the value of external applied load stress becomes 0.18 and 0.21 times the ultimate compressive stress for longitudinal and transverse orientated specimens, a shear failure appears at the specimen. The longitudinal orientated specimen found to be stronger during compressive loading and transverse orientated specimen found stronger during shear loading. Overall, the cortical bone shows weaker behaviour during shear in comparison to compressive loading. The results of this study can be applied for the bones in aquatic species like fishes, crocodiles etc. as in water, these species shall wear shear and compressive load on their bones during swimming.